Status
Completed
Phase
Not applicable
Enrollment
11
Locations
1
Results
Not posted
Publications
0
Study summary
The study evaluated the effect of serum relaxin and follicular fluid levels on the number and quality of oocytes, fertilization, the number and quality of the embryos in IVF protocols.
Relaxin is one of the promising indicators of oocyte quality and prognosis of the effectiveness of invitro fertilization protocols. The hormone plays an important role in the processes of folliculogenesis, growth and maturation of oocytes, development of embryos, implantation processes, pregnancy and childbirth. A pilot study was conducted on a group of patients undergoing infertility treatment using assisted reproductive technologies in the superovulation stimulation protocol using gonadotropin releasing hormone antagonists. Blood and follicular fluid samples were obtained on the day of transvaginal follicular puncture. The results allow us to consider the hormone relaxin as a promising predictor of the result of ovarian stimulation, the effectiveness of fertilization in IVF cycles.
Interventions
DIAGNOSTIC_TEST
Ovarian stimulation was carried out from day 3 of the menstrual cycle using a fixed protocol, with recombinant (Gonal-F, Pergoveris) or human menopausal gonadotropins (Meriofert), GnRH antagonist (ganirelix 0.25 mg). A recombinant human chorionic gonadotropin Ovitrel 250 μg or a GnRH agonist, Diferelin 0.2 mg, was used as an ovulation trigger.
Timeline
First posted
Jul 7, 2020
Study start
Dec 2, 2019
Primary completion
Feb 20, 2020
Study completion
Feb 20, 2020
Results posted
Not reported
Registry updated
Jul 7, 2020
Outcomes
Correlation between the relaxin values in follicular fluid and the number of oocytes obtained
Time frame · 10-14 days from the start of stimulation in the IVF protocol ( the day of transvaginal follicular puncture)
The level of relaxin in follicular fluid was estimated using an ELISA Kit for Relaxin 2. The range of detectable concentrations was 3.1-500 pg / ml. Higher relaxin concentrations in follicular fluid are associated with a higher number of oocytes obtained.
Correlation between the relaxin levels in follicular fluid and oocyte fertilization efficiency
Time frame · 10-14 days from the start of stimulation in the IVF protocol for relaxin. The first day after transvaginal follicular puncture for evaluation oocyte fertilization efficiency.
The level of relaxin in follicular fluid was estimated using an ELISA Kit for Relaxin 2. The range of detected concentrations was 3.1-500 pg / ml. In course of standard IVF technique the fertilization efficacy was evaluated as a ratio of double pro-nucleus zygote on the 1st day of development to obtained oocyte-cumulus complexes number. In course of ICSI the efficacy was estimated as ratio of double pro-nucleus zygote on the 1st day of development to oocyte number on MII stage on puncture day. Higher concentrations of follicular fluid relaxin are associated with a decrease efficacy of oocyte fertilization
Correlation between the relaxin levels in follicular fluid and age
Time frame · 10-14 days from the start of stimulation in the IVF protocol
The method of relaxin determining in follicular fluid and the range of determined concentrations are described previously. With increasing age, a decrease in the concentration of relaxin in the follicular fluid was noted.
Correlation between the relaxin levels in follicular fluid and BMI
Time frame · 10-14 days from the start of stimulation in the IVF protocol
The method of relaxin determining in follicular fluid and the range of determined concentrations are described previously. With increasing of BMI, a decrease in the concentration of relaxin in the follicular fluid was noted.
Eligibility
Inclusion Criteria: 1. Clinical diagnosis of Primary or Secondary infertility 2. Must be signed the voluntary informed consent to participate in the study. Exclusion Criteria: 1. The presence of IVF contra-indications. 2. Clinical diagnosis of large uterine fibroids. 3. BMI\> 35 kg / m2 4. Morphological diagnosis of endometrial hyperplastic processes 5. Clinical diagnosis of infectious. 6. Clinical diagnosis of systemic autoimmune diseases. 7. Insulin dependent diabetes 8. Oncological diseases
Study locations
Russia. Showing up to 24 locations stored in the fast local snapshot.
DOOttResearch
Saint Petersburg, Russia
Publications
No PMID-linked publications were present in this registry snapshot.
Related trials
Centro A.M.B.R.A. (Associazione Medici e Biologi per la Riproduzione Assistita) · Infertility, Female · Ovulation Induction
Early Phase 1
Not yet recruiting
189
2026-07
University of Delaware · Estrogen · Premenopause
Phase 4
Recruiting
30
2026-07
ART Fertility Clinics LLC · Healthy
Not applicable
Not yet recruiting
20
2026-05
Fundación Santiago Dexeus Font · Infertility
Phase 4
Completed
110
2026-04
Instituto Bernabeu · IVF
Phase 4
Completed
150
2026-03
Hanoi Medical University · Infertility (IVF Patients) · Embryo Morphokinetics
Not applicable
Recruiting
148
2026-02
University Hospital, Ghent · Ovarian Stimulation · Quality of Life
Phase 4
Recruiting
60
2025-12
Tam Anh TP. Ho Chi Minh General Hospital · Diminished Ovarian Reserve
Not applicable
Recruiting
730
2025-12
Related PeptideStat pages
Research pages describe evidence. Vendor pages, where available, describe independently tracked research-product listings and are not clinical recommendations.