Effect of GnRH Agonist vs GnRH Antagonist on IVF/ICSI Outcomes.

Follicular fluid Placental Growth Factor (PlGF) Concentrations:

Time frame · Immediately after oocyte retrieval (35±2 hours after hCG administration)

Follicular fluid samples will be obtained on the day of oocyte retrieval, then they will be centrifuged to eliminate cellular elements and debris. After that, the supernatants will be frozen at -80 until assayed using an Eliza kit.

Number of oocytes retrieved:

Time frame · Immediately after oocyte retrieval (35±2 hours after hCG administration)

The oocytes will be retrieved by transvaginal ultrasound-guided follicle aspiration 35±2 hours after hCG administration.

Number of Metaphase II Oocytes (MII):

Time frame · Within two hours after oocyte retrieval

The oocyte maturity will be assessed using Nikon SMZ1500 stereoscope.

Maturation Rate%:

Time frame · Within two hours after oocyte retrieval

Maturation Rate is calculated by dividing the number of mature (MII) oocytes by the number of retrieved oocytes.

Fertilization Rate%:

Time frame · 16-18 hours after microinjection

Fertilization Rate is calculated by dividing the number of obtained zygote (2PN) by the number of injected oocytes.

Cleavage Rate%:

Time frame · Day 2 after microinjection

Cleavage rate is calculated by dividing the number of cleavaged embryos by the number of zygotes (2PN).

Embryo Quality:

Time frame · Day of transfer (2 or 3 days after microinjection)

Embryos are assessed using Nikon SMZ1500 stereoscope based on ESHRE criteria (2011).

High Quality Embryos rate%:

Time frame · Day of transfer (2 or 3 days after microinjection)

High Quality Embryos rate is calculated by dividing the number of high quality embryos (Grade I) by the total number of cleavaged embryos.

Biochemical Pregnancy Rate% (Per Embryo Transfer):

Time frame · 2 weeks after embryo transfer

Biochemical pregnancy is defined as a positive serum beta-hCG pregnancy test after 2 weeks of embryo transfer. The biochemical pregnancy rate is calculated by dividing the number of women who are biochemically pregnant by the number of women who have at least 1 embryo transferred.

Clinical Pregnancy Rate% (Per Embryo Transfer):

Time frame · 3-4 weeks after embryo transfer

Clinical pregnancy is defined as the presence of a gestational sac on ultrasound after 3-4 weeks of embryo transfer. The clinical pregnancy rate is calculated as by dividing the number of women who are clinically pregnant divided by the number of women who have at least 1 embryo transferred.