Clinical Study of Bowel Preparation Before Colonoscopy

Research purpose In this study, by evaluating patients' intestinal cleanliness rate (BBPS score) and quantitative analysis of intestinal bubble under colonoscopy, three intestinal preparation schemes were respectively investigated: The effectiveness, tolerability, adverse reactions and prognosis of concentrated oral solution of sodium and potassium magnesium sulfate (experimental group 1), concentrated oral solution of sodium and potassium magnesium sulfate + linalotide (experimental group 2), PEG(compound polyethylene glycol electrolyte powder) (control group) were analyzed and compared, in order to improve the intestinal preparation cleanliness rate of colonoscopy subjects. 3\. Study the overall design 1. Research program design A single-center, single-blind, randomized controlled parallel design was adopted. 2. Entry criteria Patients aged ≥18 years and ≤80 years who need intestinal preparation before colonoscopy. 3. Study sample size In this study, PASS 15 software was used to estimate the sample size. According to previous research data, the intestinal cleanliness rates of the three groups of sodium and potassium magnesium sulfate oral concentrated solution group (test group 1), sodium and potassium magnesium sulfate oral concentrated solution + linalotide group (test group 2) and PEG group (control group) were set to be 86%, 90% and 69.36%, respectively, and the differences between the two groups and the control group were mainly compared. Considering that the total Class I error rate of multiple tests is controlled within 0.05, the bilateral test level of both tests is set as α=0.025, and the degree of assurance is 80%, and the minimum sample size of each group is calculated as 118 cases according to the equal ratio of 1:1:1. Considering a possible 20% shedding rate, it is planned to include 148 patients in each group, for a total of 444 patients. 4. Criteria for judging clinical efficacy 4.1 Main therapeutic indexes Evaluation criteria for the main efficacy indicators: The proportion of subjects with a total Boston score ≥6 and a colon score ≥2 for each segment was recorded as effective. the Boston bowel preparation scale (BBPS) can be used to evaluate the volume, morphology and mucosal clarity of stool and fluid in the bowel. The scoring criteria are as follows: 0 points: there is stool in the colon that cannot be cleared, and the mucosa can not be seen clearly, which is mostly seen in the stage without intestinal preparation; 1. Part of the intestinal mucosa was clearly displayed, while the other part of the mucosa was not clearly displayed due to feces and opaque liquid residues; 2 points: There is a small amount of stool and opaque liquid remaining in the colon; 3 points: All mucous membranes are clearly displayed, and no stool or opaque fluid remains in the colon. Rating description: 1. The colon score of each segment was 0\~3, and the total score was 0\~9: 2. The colon score of each segment was ≥2, indicating adequate intestinal preparation: 3. The total score of 6 and the score of any segment of colon \< 2 were classified as inadequate intestinal preparation. 4. The total score ≥6 and the colon score ≥2 points in each segment indicated that the intestinal preparation was adequate, otherwise it was inadequate. 4.2 Secondary efficacy indicators Evaluation criteria of secondary curative effect indicators: those who scored 1 or 2 points (i.e., \> 0 points) under colonoscopy were recorded as satisfied. Colonoscopic evaluation of intestinal bubbles, the scoring criteria are as follows: 0 points: there are many bubbles in the intestinal cavity, requiring a lot of water to rinse repeatedly after observation; 1 point: there are few bubbles in the intestinal cavity, which need to be observed after a small amount of washing; 2 points: There are almost no bubbles in the intestinal cavity, and it can be observed well without rinsing. 4\. Case selection 1. Study subjects: patients aged ≥18 years and ≤80 years who need intestinal preparation before colonoscopy. 2. Inclusion criteria: 1\) Signed written informed consent; 2) Age 18-80 years old, gender is not limited; 3) The subjects could follow the follow-up plan, objectively describe the symptoms, and cooperate with the completion of the scale; 4) Non-lactating pregnant women and no pregnancy plan during the test; 5) Do not participate in any clinical trials for 3 months before and during the trial; 6) Participate voluntarily and sign informed consent. 3. Exclusion criteria 1. NYHA cardiac function grade III or IV; 2. Unstable angina pectoris in the convalescence period of acute myocardial infarction or in the near future; 3. have serious liver and kidney function diseases; 4. Suspected gastrointestinal obstruction or gastrointestinal perforation; 5. Pregnant or lactating women; 6. Mental illness or physical dysfunction can not cooperate with the examination; 7. People who are allergic to intestinal preparation drugs. 8. Patients who did not undergo colonoscopy after bowel clearing due to their own reasons; 9. Patients who can not tolerate general colonoscopy and terminate the examination; 10. Patients who requested withdrawal from the study. 5. Clinical trial procedures <!-- --> 1. Overview: The subjects signed informed consent and were randomly assigned to complete intestinal preparation according to the requirements of the corresponding drugs, and underwent colonoscopy and routine postoperative treatment. The subjects were asked to receive regular telephone follow-up or outpatient follow-up, receive recent information, imaging examination, laboratory examination and other methods to evaluate the effects of the drugs, collect complete medical records and complete statistics. 2. Treatment grouping and random allocation: In this study, biostatistics professionals used the block randomization method with variable block length to generate a random assignment sequence, and the enrolled subjects were randomly assigned to three groups in equal proportion (1:1:1). The three treatment groups were: (1) sodium and potassium magnesium sulfate solution group: 3L oral concentrated solution of sodium and potassium magnesium sulfate. At 18:00 1d before the inspection, dissolve a package of contents into 200ml with water, and drink 250ml of water every half hour after that, drinking a total of 6 cups (that is, 1.5L); On the day of the examination (6 hours before the colonoscopy) repeat the same medication as the previous evening. (2) Magnesium sulfate sodium potassium solution + linaclotide group: 3L sodium magnesium sulfate potassium oral concentrated solution + linaclotide 290μg. One tablet of linalotide was taken orally at 18:00 one day before the examination, and then two packets of contents were dissolved into 300ml with water 6 hours before the colonoscopy. After that, 500ml of water was drunk every half hour, for a total of 6 cups. (3) PEG (Compound polyethylene glycol electrolyte powder) group: 3LPEG. At 18:00 1d before the inspection, dissolve a package of contents into 200ml with water, and drink 250ml of water every half hour after that, drinking a total of 6 cups; On the day of the examination (6 hours before the colonoscopy) repeat the same medication as the previous evening. In practice, the mobile phone-based "Ratjin" wechat mini program was used to achieve random allocation of the three treatment groups (the specific randomization scheme will be developed separately). When the subjects sign the informed consent, and the subjects meeting the test enrollment conditions need to be randomized after screening, the authorized researcher will input the relevant information of the patients in the mobile phone client, and after the approval and confirmation of the designated random administrator, the assigned group will be immediately fed back to the mobile phone, and the researcher will implement the corresponding treatment for the patients according to the randomly assigned group. This method can generate random assignment sequence in advance, realize random assignment concealment completely, and prevent selection bias effectively. 3. Preparation before treatment: 2 days before colonoscopy, the three groups were forbidden to eat high-fiber vegetables, dragon fruit and other seeded fruits, and should mainly eat low-fiber and low-residue foods, and eat dinner before 6 PM, and then fast; Check for semi-liquid or liquid food the night before, and check for fasting and no drinking on the day. 4. Post-treatment: In order to replace fluid loss during the examination, the patient subsequently drinks an adequate amount of fluid to maintain adequate hydration. Seek medical attention if severe or persistent diarrhea occurs. 5. Participants in the test and division of labor 1) 3 experienced endoscopists and several experienced endoscopic nurses cooperated with the doctor to complete the use of endoscopic instruments. 2\) 2 researchers observed and recorded, and carried out related work: A. After colonoscopy, the researchers recorded and statistically analyzed the intestinal cleanliness of the subjects, the content of bubbles in the intestinal cavity, the location, size and number of detected polyps, and adenomatous polyps. B. Record, observe and record the occurrence of adverse reactions (such as the occurrence of nausea, vomiting, headache, dizziness, abdominal pain, abdominal distension, etc.); C. Assist with various incidents. 6. Follow-up procedure Observation node and follow-up procedure: After colonoscopy, the patients were asked about their satisfaction with the taste of laxatives, whether they were satisfied with this group of bowel cleansing methods, adverse reactions (nausea, vomiting), etc. 7\. Observation indicators 1. General information: age, sex, ethnicity, occupation, height, weight, medical history, medication history, contact address and telephone number 2. Main outcome measures: Patients' intestinal cleanliness rate (BBPS…